Amita, Ajit and Santhoshkumar, T. R and Manesh , Senan (2020) Generation of niche tuned antifibrotic fibroblasts and non-viralmediated endothelial commitment using adipose stem cells fordermal graft development. Journal of Biomedical Materials Research Part B. pp. 1-13. ISSN 1552-4981

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Abstract

Cell-based skin substitute generation has seen considerable development. Combiningsynthetic scaffolds with biomimetic fibrin does direct both exogenous and endogenousstem cell differentiation, addressing needs for reliable tissue engineering. However, lackof immediate vasculature within implantable grafts remains critical for its sustenance andintegration. Multipotency, high proliferationpotential, ability to release multiple growthfactors (GFs), and autologous availability highlight the use of human adipose derivedmesenchymal stem cells (hADMSCs) in tissue-engineered dermal grafts (TEDG) construc-tion. However, hADMSCs' insufficiency to independently establish angiogenesis withintissue constructs demands improvement of stem cell application for dermal graft survival.Approaches to harness microenvironmentallysensitive paracrine interactions couldimprove the angiogenic efficiency of hADMSCs within TEDG. This study conceptualizeda fibrin-based niche, to direct hADMSCs toward a nonfibrotic fibroblast commitmentand incorporation of bioengineered hADMSCs,specifically releasing potent angiogenicfactors within TEDG. Coexistence of tuned fibroblast and endothelial lineage committedcells contributed to well-regulated extracellular matrix formation and prevascularization.Adequate cell proliferation; sustained transient release of angiogenic GFs till 20 days;directed dermal, endothelial, fibroblast, and vascular smooth muscle cell differentiation;and favored elastin and collagen deposition were achievedin vitro. In conclusion, specificniche composition and employment of bioengineered hADMSCs favor implantableTEDG construction.

Item Type:	Article
Uncontrolled Keywords:	adult stem cells, fibrin-based niche, non-viral transfection, prevascularization, tissue-engineered dermal grafts
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	13 Jan 2021 07:12
Last Modified:	13 Jan 2021 07:12
URI:	http://rgcb.sciencecentral.in/id/eprint/1009

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