Shikha T, Rames and Navyasree, Kolaparamba V. and Sneha, Sah and Anjitha, B. Ashok and Nishada , Qathoon and Umasankar, P K. (2021) BMP2K phosphorylates AP-2 and regulates clathrin-mediated endocytosis. Traffic, 22 (11). pp. 377-396. ISSN 1600-0854

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Abstract

Phosphorylation of the central adaptor protein complex, AP-2 is pivotal for clathrin-mediated endocytosis (CME). Here, we uncover the role of an uncharacterized kinase (BMP-2 inducible kinase—BMP2K) in AP-2 phosphorylation. We demonstrate that BMP2K can phosphorylate AP-2 in vitro and in vivo. Functional impairment of BMP2K impedes AP-2 phosphorylation leading to defects in clathrin-coated pit (CCP) morphology and cargo internalization. BMP2K engages AP-2 via its extended C-terminus and this interaction is important for its CCP localization and function. Notably, endogenous BMP2K levels decline upon functional impairment of AP-2 indicating AP-2 dependent BMP2K stabilization in cells. Further, functional inactivation of BMP2K in zebrafish embryos yields gastrulation phenotypes which mirror AP-2 loss-of-function suggesting physiological relevance of BMP2K in vertebrates. Together, our findings propose involvement of a novel kinase in AP-2 phosphorylation and in the operation of CME.

Item Type:	Article
Subjects:	Interdisciplinary Biology
Depositing User:	Central Library RGCB
Date Deposited:	07 Jan 2022 07:02
Last Modified:	07 Jan 2022 07:02
URI:	http://rgcb.sciencecentral.in/id/eprint/1075

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