Sreeja, Jamuna S and Athira, Jyothy and Suparna, Sengupta (2021) α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins. Genes, 12 (5). ISSN 2073-4425

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Abstract

Cortical cytoskeletal proteins are significant in controlling various cellular mechanisms such as migration, cell adhesion, intercellular attachment, cellular signaling, exo- and endocytosis and plasma membrane integrity, stability and flexibility. Our earlier studies involving in vitro and ex vivo approaches led us to identify certain undiscovered characteristics of α-fodrin, a prominent cortical protein. The conventional functions attributed to this protein mainly support the plasma membrane. In the present study, we utilized a global protein expression analysis approach to detect underexplored functions of this protein. We report that downregulation of α-fodrin in glioblastoma cells, U-251 MG, results in upregulation of genes affecting the regulation of the cytoskeleton, cell cycle and apoptosis. Interestingly, certain key microtubule kinesins such as KIF23, KIF2B and KIF3C are downregulated upon α-fodrin depletion, as validated by real-time PCR studies.

Item Type:	Article
Uncontrolled Keywords:	fodrin, spectrin, kinesins, LC-MS, proteomics
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	11 Jan 2022 08:10
Last Modified:	11 Jan 2022 08:11
URI:	http://rgcb.sciencecentral.in/id/eprint/1085

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