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Reshma, K Mukesh and Azeem , Abdul Kalam and Joydeep , Nag and Vishnu , Sunil Jaikumar and Umerali, Kunnakkadan and Nisha, Asok Kumar and Sreenath , Muraleedharan Suma and Arumugam , Rajavelu and John , Bernet Johnson (2021) Chandipura virus induces cell death in cancer cell lines of human origin and promotes tumor regression in vivo. Molecular therapy oncolytics. pp. 254-265. ISSN 2372-7705

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Chandipura virus (CHPV) is an emerging human pathogen of great clinical significance. In this study, we have investigated the susceptibility pattern of both normal and cancer cell lines of human origin to wild-type (wt) CHPV in order to explore the possibility of developing CHPV as an oncolytic vector (OV). Marked cytopathic effect along with enhanced virus output was observed in cancer cell lines (HeLa, A549, U-138, PC-3, and HepG2) in comparison to normal human adult dermal fibroblast (HADF) cells. At an MOI of 0.1, cancer cell lines were differentially susceptible to CHPV, with cells like HeLa and U-138 having pronounced cell death, while the PC-3 were comparatively resistant. All cell lines used in the study except U-138 restricted CHPV infection to varying degrees with IFN-β pre-treatment and supplementation of interferon (IFN) could neither activate the IFN signaling pathway in U-138 cells. Finally, U-138 tumor xenografts established in non-obese diabetic severe combined immunodeficiency (NOD/SCID) mice showed significant delay in tumor growth in the CHPV-challenged animals. Thus, targeted cytopathic effect in cancer cells at a very low dose with restricted replication in normal cells offers a rationale to exploit CHPV as an oncolytic vector in the future.

Item Type: Article
Uncontrolled Keywords: Chandipura virus, cytopathic property, oncolytic vector, interferon-β, tumor growth and regression, U-138 glioma cells, xenograft model, anti-viral response, virus induced cytotoxicity
Subjects: Pathogen Biology
Depositing User: Rgcb Library
Date Deposited: 17 Feb 2022 08:42
Last Modified: 17 Feb 2022 08:42
URI: http://rgcb.sciencecentral.in/id/eprint/1122

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