Kuruvilla , L and Santhosh Kumar, TR and Panicker, S R and Kartha, C. C (2014) Vascular Endothelial Growth Factor Gene Transfection in Endocardial Endothelial Cells Promote Capillary Growth in Vitro. Journal of Cardiovascular Disorders, 1 (1). ISSN 2379-7991

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Abstract

Different types of cells have been employed both in in vitro and in vivo studies for revascularization of ischemic myocardium. Whether Endocardial Endothelial Cells (EEC) lying adjacent to the myocardium can be used in gene therapy for successful revascularization of ischemic myocardium remains unexplored. Here we present evidence for the frst time that EECs can be made to take up Vascular Endothelial Growth Factor (VEGF) gene with consequent VEGF protein synthesis to promote angiogenesis in vitro. Methods and Results: Immortalized EECs (iEEC) with stable h TERT tranfection were transfected with VEGF gene. The iEECs, Rat Aortic Endothelial Cells (RAECs) and VEGF transfected cells were seeded in various combinations onto matrigel and the number of tubes counted using tube formation assay. Cocultures of RAECs and VEGF transfected EECs gave the maximum number of tubes (24.6 ± 3.7) and also the maximum tube length (334 ± 45 µm) within the shortest period of time. iEECs alone did not show any tube formation whereas iEECs with VEGF gene formed a few incomplete tubes. Conclusion: These results confrm that VEGF gene transfection in EECs can promote capillary growth in vitr

Item Type:	Article
Uncontrolled Keywords:	Endocardium; Endothelium; VEGF; Angiogenesis
Subjects:	Disease Biology
Depositing User:	Central Library RGCB
Date Deposited:	09 Jan 2017 04:52
Last Modified:	22 Apr 2019 09:29
URI:	http://rgcb.sciencecentral.in/id/eprint/124

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