Suboj, Babykutty and Priya , Srinivas (2012) Nimbolide Retards Tumor Cell Migration, Invasion, and Angiogenesis by Downregulating MMP-2/9 Expression Via Inhibiting ERK1/2 and Reducing DNA-Binding Activity of NF-kB in Colon Cancer Cells. Molecular carcinogenesis, 51 (6). pp. 475-90. ISSN 1098-2744

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Abstract

Nimbolide, a plant-derived limonoid has been shown to exert its antiproliferative effects in various cell lines. We demonstrate that nimbolide effectively inhibited proliferation of WiDr colon cancer cells through inhibition of cyclin A leading to S phase arrest. It also caused activation of caspase-mediated apoptosis through the inhibition of ERK1/2 and activation of p38 and JNK1/2. Further nimbolide effectively retarded tumor cell migration and invasion through inhibition of metalloproteinase-2/9 (MMP-2/9) expression, both at the mRNA and protein level. It was also a strong inhibitor of VEGF expression, promoter activity, and in vitro angiogenesis. Finally, nimbolide suppressed the nuclear translocation of p65/p50 and DNA binding of NF-κB, which is an important transcription factor for controlling MMP-2/9 and VEGF gene expression.

Item Type:	Article
Uncontrolled Keywords:	nimbolide; colon cancer; MMP-2/9; ERK1/2; invasion
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	17 Feb 2017 03:47
Last Modified:	17 Feb 2017 03:47
URI:	http://rgcb.sciencecentral.in/id/eprint/245

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