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Aswathy, Ammothumkandy and Tessy Thomas , Maliekal (2016) CD66 and CD49f expressing cells are associated with distinct neoplastic phenotypes and progression in human cervical cancer. European Journal of Cancer, 60. pp. 166-78. ISSN 0959-8049

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BACKGROUND: In this study, building on our recent work identifying a subset of CD66+ve cells with distinctive tumourigenic properties in human cervical cancers, we examine patterns of expression and function of these cells; to generate insights into the process of metastasis. METHODS: Our broad approach in this study has been to compare the expression and function of two subsets marked by CD66 and CD49f. We use a combination of histopathology, immunostaining and flow cytometry, functional analysis of an established cervical cancer cell line and a retrospective analysis of a cohort of cervical cancer. RESULTS: We noted CD66 expression associated with clusters of cells which are spindle shaped, SMA+ve, podoplanin+ve, phalloidin high, fibronectin high, plakoglobin low, ki67-ve and CK10+ve at the migratory phase along with features of partial EMT. Further, TGFβ1 a well known regulator of EMT, positively correlated with CD66 expression. The additional CD49f+ve subset at the leading invading front of migration was SMA-ve, phalloidin low, fibronectin low, plakoglobin high, Ki67+ve and CK14+ve. These data are consistent with a role for CD66 cells in metastatic invasion with a collective cell migration process co-opting the CD49f subset. Our retrospective analysis of a cohort is consistent with a role for CD66 in metastasis. However, the broad analysis of CD66, CD49f and TGFβ1 expression with patterns of overall survival points to a possible protective effect particularly for local recurrences. Hence, future studies focussing on potential heterogeneity within the CD66 subset along with the possible role of isoforms and intra-cellular roles would be essential.

Item Type: Article
Uncontrolled Keywords: CD49f; CD66; Cervical cancer; Migration; Tumourigenesis
Subjects: Cancer Research
Depositing User: Central Library RGCB
Date Deposited: 20 Mar 2017 09:17
Last Modified: 20 Mar 2017 09:17
URI: http://rgcb.sciencecentral.in/id/eprint/309

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