Emilie, Layre and Sathish , Mundayoor (2011) Deciphering sulfoglycolipids of Mycobacterium tuberculosis. Journal of lipid research, 52 (6). ISSN 1539-7262

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Abstract

For 4 decades, in vivo and in vitro studies have suggested that sulfoglycolipids (SGLs) play a role in the virulence or pathogenesis of the tubercle bacilli. However, the SGL structure and biosynthesis pathway remain only partially elucidated. Using the modern tools of structural analysis, including MALDI-time-of-flight MS, MS/MS, and two-dimensional NMR, we reevaluated the structure of the different SGL acyl (di-, tri-, and tetra-acylated) forms of the reference strain Mycobacterium tuberculosis H37Rv, as well as those produced by the mmpL8 knockout strains previously described to intracellularly accumulate di-acylated SGL. We report here the identification of new acyl forms: di-acylated SGL esterified by simple fatty acids only, as well as mono-acylated SGL bearing a hydroxyphthioceranoic acid, which were characterized in the wild-type strain. In a clinical strain, a complete family of mono-acylated SGLs was characterized in high abundance for the first time. For the mmpL8 mutant, SGLs were found to be esterified i) by an oxophthioceranoic acid, never observed so far, and ii) at nonconventional positions in the case of the unexpected tri-acylated forms. Our results further confirm the requirement of MmpL8 for the complete assembly of the tetra-acylated forms of SGL and also provide, by the discovery o

Item Type:	Article
Uncontrolled Keywords:	acylation, biosynthesis, glycolipids, lipids, mycobacteria, structure elucidation, sulfolipids
Subjects:	Mycobacterium Research
Depositing User:	Central Library RGCB
Date Deposited:	01 May 2017 07:00
Last Modified:	01 May 2017 07:00
URI:	http://rgcb.sciencecentral.in/id/eprint/361

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