preetha, K and sanil, george (2014) First record of Granulifusus poppei (Mollusca: Fasciolariidae) from Indian coast. Marine Biodiversity Records, 7.

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Abstract

Fasciolariids, the tulip snails or spindle snails, represented in the molluscan family Fasciolariidae, constitute an important component of the molluscan fauna in shallow coastal waters (Petuch, 1987), especially on sandy substrates. These carnivorous snails are widely distributed in tropical and temperate waters of oceans at sub-tidal depths (Harasewych, 1998; Snyder, 2003). The family comprises more than 100 genera grouped under three main subfamilies: Fasciolariinae, Fusininae and Peristerninae (Bouchet, 2014). Kuroda & Habe (1952) described the genus Granulifusus and it currently accommodates 27 valid species worldwide (Bouchet, 2014). The exact range of geographical distribution of all the species is not known and the genus Granulifusus is thus far not reported from the Indian coast. This paper records the occurrence of Granulifusus poppei Delsaerdt, 1995 from the Indian coast and provides the sequence data of the mitochondrial gene cytochrome c oxidase 1 (CO1) of the species. M A T E R I A L S A N D M E T H O D S Three specimens (shell length 35–41 mm; shell width 13–16 mm; aperture length 11–12 mm; aperture width 4–6 mm) were collected by bottom trawlers at an average depth of 100 m off the Kerala coast (9o31′N and 75o33′E), India. Morphometric measurements were recorded to the nearest millimetre using a digital Vernier calliper. Two specimens are deposited in the museum collections of the Department of Aquatic Biology and Fisheries, University of Kerala, India (Voucher Numbers DABF-UOK/GAS 2-3). The third specimen was preserved in 95% ethanol for molecular analysis. The material was identified following the criteria of Delsaerdt (1995) and Robin (2008). Total DNA was extracted from the muscle tissue of G. poppei using DNeasy Blood and Tissue Kit (QIAGEN). The mitochondrial gene CO1 was amplified using the universal primers (Folmer et al., 1994) in a 25 ml reaction volume with Taq PCR master mix (QIAGEN) and template DNA using the thermal cycler (Eppendorf). The PCR products were purified with ExoSAP-IT (USB) and sequenced in forward and reverse directions using the corresponding PCR primers and Big Dye Terminator V.3.1 Cycle Sequencing Kit (Applied Biosystems, Inc.) in an ABI 3730 capillary sequencer. Intra-specific distance between the species of the Granulifusus genus was estimated using the Kimura 2-parameter distance model of MEGA (Version 6.0) Package (Tamura et al., 2013).

Item Type:	Article
Uncontrolled Keywords:	Fasciolariidae, Kerala, Granulifusus, distribution, Indian Ocean
Subjects:	Environmental Biology
Depositing User:	Central Library RGCB
Date Deposited:	14 Jul 2017 06:32
Last Modified:	22 Apr 2019 09:28
URI:	http://rgcb.sciencecentral.in/id/eprint/403

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