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Sunil V. , Joseph and Biljo V., Joseph and Leny , Jose and Sathish , Mundayoor (2013) Bioconversion of Sodium Dodecyl Sulphate to Rhamnolipid by Pseudomonas aeruginosa: A Novel and Cost-Effective Production Strategy. Applied biochemistry and biotechnology, 169 (2). pp. 418-430. ISSN 1559-0291

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Abstract

The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Pseudomonas aeruginosa S15 capable of concomitant SDS degradation and biosurfactant synthesis. SDS-based rhamnolipid production was proved on SDS minimal agar plates using cetyl trimethylammonium bromide– methylene blue method and optimised in SDS-based minimal salt (SBS) medium. SDS proved to be an ideal carbon source for rhamnolipid synthesis with a high substrate to product conversion rate yielding 6.9 g/l of rhamnolipids from 1 g/l SDS in 5 days. Fast atom bombardment mass spectroscopy analysis of the purified biosurfactant proved the presence of mono- and di-rhamnolipids, viz., Rha-C10-C10, Rha-C10-C12 and Rha-Rha-C10-C10 with surface active properties. The secreted rhamnolipids were not utilised by S15 as a carbon source, but it caused a dispersion of bacterial biofilms in SBS medium. To the best of our knowledge, this is the first report on bioconversion of synthetic detergent to biodetergent. This SDS-based novel methodology presents a more economised mode of rhamnolipid synthesis utilising SDS as sole carbon source

Item Type: Article
Uncontrolled Keywords: Biosurfactant Bioremediation Sodium dodecyl sulphate Detergent Biofilm
Subjects: Mycobacterium Research
Depositing User: Rgcb Library
Date Deposited: 21 Jul 2017 10:26
Last Modified: 21 Jul 2017 10:26
URI: http://rgcb.sciencecentral.in/id/eprint/419

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