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Saleem, Shaikh and Rajendran, Ravenndranath and Moinak, Banerjee and Anna, Joseph and Pramod, Jahgirdar (2012) Evidence for transforming growth factor–beta 3 gene polymorphism in nonsyndromic cleft lip and palate patients from indian sub-continent. Medicina oral, patología oral y cirugía bucal., 17(2). ISSN 1698-6946

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Abstract

Objectives: Orofacial clefts are major human birth defects with complex etiology. Previous studies have proposed Transforming growth factor - beta 3 (TGF-β3) gene as a key player in contributing to non-syndromic cleft lip and palate, however none of the studies have yet included Indian population. Hence this study was designed to detect TGF-β3 gene polymorphism in nonsyndromic cleft lip and palate patients from Indian population which is genetically distinct from previously studied populations. Study Design: Peripheral blood samples of forty non-syndromic cleft lip and palate patients and forty unaffected individuals were collected for a case – control study design. Ethical clearance from the institutional review board and informed consent from all subjects was obtained. DNA extracted from the cases and controls was amplifed using polymerase chain reaction (PCR) with TGF-β3 specifc primers. The obtained fragments were sequenced and TGF-β3 gene polymorphisms were assessed based on the number of CA repeats. Results: Chi –square test was used to compare the case and control groups. Results showed a signifcant difference in the number of CA repeats between the case and the control groups (p=0.01). Conclusion: This study confrms the crucial role of TGF-β3 in the fusion of palatal shelves during development and further, provides novel evidence of TGF-β3 gene polymorphism in the etiology of nonsyndromic cleft lip and palate in Indian subpopulation.

Item Type: Article
Uncontrolled Keywords: Orofacial clefts, nonsyndromic cleft lip with/without cleft palate, TGF- β 3, Polymerase chain reaction, gene polymorphism
Subjects: Molecular Neurobiology
Depositing User: Rgcb Library
Date Deposited: 22 Dec 2016 02:58
Last Modified: 22 Dec 2016 05:48
URI: http://rgcb.sciencecentral.in/id/eprint/44

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