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Vineeth Kumar, TP and Asha, R and G , Shyla and Sanil, George (2017) Identification and characterization of novel host defense peptides from the skin secretion of the fungoid frog, Hydrophylax bahuvistara (Anura: Ranidae). Chemical biology & drug design. pp. 1-10. ISSN 1747-0285

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Abstract

Two novel peptides (brevinin1 HYba1 and brevinin1 HYba2) were identified from the skin secretion of the frog Hydrophylax bahuvistara, endemic to Western Ghats, India, and their amino acid sequences were confirmed using cDNA cloning and LC/MS/MS. Antibacterial, hemolytic, and cytotoxic activities of brevinin1 peptides and their synthetic analogs (amidated C-terminus) were investigated and compared. All the peptides except the acidic forms showed antibacterial activity against all tested Gram-positive and Gram-negative bacteria. They exhibited low hemolysis on human erythrocytes and showed potent cytotoxic activity against Hep 3B cancer cell line. Upon amidation, the peptides showed increased activity against the tested microbes without altering their hemolytic and cytotoxic properties. The study also emphasizes the need for screening endemic amphibian fauna of Western Ghats, as a potential source of host defense peptides with possible therapeutic applications in the future.

Item Type: Article
Uncontrolled Keywords: C-terminal amidation; Western Ghats; amphibia; antibacterial activity; brevinin1; hemolysis; host defense peptide
Subjects: Molecular Ecology
Depositing User: Rgcb Library
Date Deposited: 03 Nov 2017 06:08
Last Modified: 03 Nov 2017 06:08
URI: http://rgcb.sciencecentral.in/id/eprint/492

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