Vini, Ravindran and Juberiya M , Azeez and Sreeja, Sreeharshan (2016) Evidence of Pomegranate Methanolic Extract In Antagonizing the Endogenous SERM, 27- Hydroxycholesterol. IUBMB life, 68 (2). pp. 116-21. ISSN 1521-6551

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Abstract

The direct relationship between obesity and breast cancer has been elucidated recently with the identification of a cholesterol derivative 27-hydroxycholesterol (27HC), an endogenous SERM that can act through estrogen receptor (ER)-mediated mechanisms. Our recent research shed light on the possible SERM-like property of methanol extract of pericarp of pomegranate (PME) by using human breast (MCF-7, MDA-MB-231), endometrial (HEC-1A), cervical (SiHa, HeLa), ovarian (SKOV3) cancer cell lines, normal breast fibroblasts (MCF-10A) and also by in vivo models (ovariectomized Swiss albino mice). Our findings demonstrated that PME binds to ER and downregulates the Estrogen response elements (ERE)-mediated transcription in breast cancer cells without being agonistic in the uterine endometrium and has cardioprotective effects comparable to that of 17-β-estradiol. This preliminary work indicates the ability of PME to antagonize the activity of 27HC. We hypothesize that PME can compete with 27HC for ERα and reduce 27HC-induced proliferation of MCF-7 cells. Relevant estrogen-regulated genes such as pS2, PR and ERα were checked to evaluate the ability of PME to abrogate 27HC-induced genes. This study is significant, being the first report describing that bioactive components of the methanolic extract of pericarp of PME, a proven SERM could plausibly compete for 27HC

Item Type:	Article
Uncontrolled Keywords:	27-hydroxycholesterol; SERM; pomegranate; obesity; breast cance
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	08 Feb 2018 10:55
Last Modified:	08 Feb 2018 10:55
URI:	http://rgcb.sciencecentral.in/id/eprint/498

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