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Arjun, J. K. and Aneesh, B. and Kavitha, T. and Hari Krishnan, K. (2016) Therapeutic L-Asparaginase Activity of Bacteria Isolated from Marine Sediments. International Journal of Pharmaceutical Sciences and Drug Research, 8 (4). pp. 229-234. ISSN 0975-248X

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Abstract

L-Asparaginase, a therapeutic enzyme used in lymphoblastic leukemia and lymphosarcoma chemotherapy which is derived mostly from the bacterial sources Escherichia coli and Erwinia sp. The long term administration of the drug leads to the development of resistant tumours and anaphylactic shock in certain individuals. Hence serologically different L-Asparaginase from novel microbial sources with enhanced therapeutic potential and immunological characteristics is an essential requirement. The marine bacteria having diverse range of potential enzymes might be a source for L-Asparaginase with novel properties, which are still unexplored. In this study, we have screened marine bacteria isolated from the coastal regions of Kerala which showed both intra and extra cellular L-Asparaginase activity. Bacillus sp. (Accession no KF142395) was found to have the highest extracellular enzyme activity (2.31 IU/ml) while Shewanella sp. (Accession no KF142390) showed maximum intracellular Asparaginase activity (2.16 IU/ml).The crude extracellular enzyme preparation from Bacillus sp. had cytotoxic effect on HL60 cell line with an IC50 value of 12.5μg/ml.

Item Type: Article
Uncontrolled Keywords: L-Asparaginase, 16S rRNA, Antineoplastic, Therapeutic-enzyme, MTT assay.
Subjects: Environmental Biology
Depositing User: Rgcb Library
Date Deposited: 09 Feb 2018 05:39
Last Modified: 12 Apr 2019 07:42
URI: http://rgcb.sciencecentral.in/id/eprint/500

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