Jayesh , Antony and Minakshi , Saikia and Vinod., V and Lekshmi. R, Nath and Shabna, A and Harsha , Chandran and Sophia Margaret, Joseph and Elizabeth Jayex , Panakkal and Sriramya, I. V and Sridivya, I. V and Ruby John , Anto (2015) DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria. Scientific reports. ISSN 2045-2322

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Abstract

Wrightia tinctoria is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F5, from the dichloromethane extract of W. tinctoria leaves against malignant melanoma. DW-F5 exhibited antimelanoma activities, preventing metastasis and angiogenesis in NOD-SCID mice, while being nontoxic in vivo. The major pathways in melanoma signaling mediated through BRAF, WNT/β-catenin and Akt-NF-κB converging in MITF-M, the master regulator of melanomagenesis, were inhibited by DW-F5, leading to complete abolition of MITF-M. Purification of DW-F5 led to the isolation of two cytotoxic components, one being tryptanthrin and the other being an unidentified aliphatic fraction. The overall study predicts Wrightia tinctoria as a candidate plant to be further explored for anticancer properties and DW-F5 as a forthcoming drug formulation to be evaluated as a chemotherapeutic agent against malignant melanoma.

Item Type:	Article
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	19 Feb 2018 10:52
Last Modified:	19 Feb 2018 10:52
URI:	http://rgcb.sciencecentral.in/id/eprint/515

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