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Aravind , Madhavan and Raveendran, Sindhu and Binod, Parameswaran and Rajeev K. , Sukumaran and Ashok , Pandey (2017) Metagenome Analysis: a Powerful Tool for Enzyme Bioprospecting. Applied biochemistry and biotechnology, 183 (2). pp. 636-651. ISSN 1559-0291

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Abstract

Microorganisms are found throughout every corner of nature, and vast number of microorganisms is difficult to cultivate by classical microbiological techniques. The advent of metagenomics has revolutionized the field of microbial biotechnology. Metagenomics allow the recovery of genetic material directly from environmental niches without any cultivation techniques. Currently, metagenomic tools are widely employed as powerful tools to isolate and identify enzymes with novel biocatalytic activities from the uncultivable component of microbial communities. The employment of next-generation sequencing techniques for metagenomics resulted in the generation of large sequence data sets derived from various environments, such as soil, the human body and ocean water. This review article describes the state-of-the-art techniques and tools in metagenomics and discusses the potential of metagenomic approaches for the bioprospecting of industrial enzymes from various environmental samples. We also describe the unusual novel enzymes discovered via metagenomic approaches and discuss the future prospects for metagenome technologies.

Item Type: Article
Uncontrolled Keywords: Bioprospecting Metagenomics Metatranscriptomics Enzyme
Subjects: Mycobacterium Research
Depositing User: Rgcb Library
Date Deposited: 11 Jun 2018 10:35
Last Modified: 11 Jun 2018 10:35
URI: http://rgcb.sciencecentral.in/id/eprint/610

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