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Arjun, Jaya Kumar and Aneesh, Balakrishna Pillai and Kavitha, Thulasi and Harikrishnan, Kumarapillai (2018) Characterization of a novel asparaginase from soil metagenomic libraries generated from forest soil. Biotechnology letters, 40 (2). 343-348 . ISSN 1573-6776

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Abstract

OBJECTIVES: To screen soil metagenomic libraries for novel enzymes with enhanced activities. RESULTS: To screen soil metagenomic libraries for novel enzymes with enhanced activities. A novel L-asparaginase was identified from forest soil metagenome and its characteristics were studied. The purified protein had a specific activity of 696 IU mg-1 and optimum activity at pH 7 and 35 °C. Enhanced enzyme activities were observed in the presence of Mg2+, Ca2+ and K+. The Km value, 2 mM, and enzyme specificity constant 7.7 mM-1s-1 indicated that the recombinant enzyme has good substrate affinity to L-asparagine compared with commercially-available Escherichia coli asparaginase. The IC50 value of 0.78 µg ml-1 (0.47 IU ml-1) was observed with HL60 cell line and 0.39 µg ml-1(0.23 IU ml-1) with MOLT-3 and MOLT-4 cell lines, which is better than that of commercially-available drugs. CONCLUSION: The soil metagenome derived L-asparaginase with enhanced activities could be a potential candidate to develop as a drug in Acute Lymphoblastic Leukemia (ALL) therapy.

Item Type: Article
Uncontrolled Keywords: Acute Lymphoblastic Leukemia Anticancer l-Asparaginase Metagenomics Therapeutic enzyme
Subjects: Environmental Biology
Depositing User: Rgcb Library
Date Deposited: 06 Jul 2018 07:10
Last Modified: 06 Jul 2018 07:10
URI: http://rgcb.sciencecentral.in/id/eprint/639

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