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Mahesh S., Krishna and A., Aneesh Kumar and K. A., Abdul Jaleel (2018) Time-dependent alterations in mRNA, protein and microRNA during in vitro adipogenesis. Molecular and cellular biochemistry. pp. 1-8. ISSN 1573-4919

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Adipogenesis is a complex biological process involving synchronised interplay of different nuclear receptors. Aberration in the process leads to obesity and associated disorders. Addressing the complexity of molecular mechanisms, we worked on characterising the changes in NR1C3/PPARγ-, NR1H3/LXRα- and NCoAs/SRCs-associated microRNA, genes and proteins during different time points of adipogenesis. Glucose uptake of differentiating cells was checked at selected time points with FACS. Observations on gene expression pattern pointed a correlation in adipogenic-related genes and increased expression of PPARγ, but not LXRα. Western blot experiments also supported the gene expression pattern. MicroRNAs that vary during adipogenesis was selected using bioinformatics tools and database. Real-time PCR-based experiments showed a change in the expression of mmu-mir-23a-3p, 206-3p, 17-3p, 126a-3p and 1a-3p. Mmu-mir-23a-3p showed a gradual decrease in expression corresponding to the progression of adipogenesis. MicroRNA 23a-3p and 1a-3p showed positive association to the mRNA levels of NCoA1 and 3. Overall, the study elaborates time-dependent variations in nucleic acid and protein expression during adipogenesis in accordance to fatty acid and glucose metabolism.

Item Type: Article
Uncontrolled Keywords: Adipogenesis PPARγ LXRα MicroRNA SRC Mmu-mir-23a-3p Mmu-mir-1a-3p
Subjects: Cardiovascular Diseases And Diabetes Biology
Depositing User: Rgcb Library
Date Deposited: 06 Jul 2018 08:41
Last Modified: 06 Jul 2018 08:41
URI: http://rgcb.sciencecentral.in/id/eprint/640

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