SANIL, GEORGE and SUJITH, V.G and MEENAKSHI, K (2011) IDENTIFICATION OF UNKNOWN TADPOLES USING DNA BARCODE SEQUENCES. Recent Advances in Biodiversity of India. pp. 293-295.

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Abstract

The use of DNA barcodes has been proposed recently as a tool for identification of the species in many diverse groups of animals (Hebert et al., 2003a; www.barcoding.si.edu., 2005).The mitochondrial gene cytochrome c oxidase subunit 1 (COI) is reported to be the most conserved gene for DNA barcoding owing to its advantages such as maternal lineage, lack of recombination, lack of indels and higher mutation rates (Hebert et al., 2005; Hajibabaei et al., 2006). One potential application of DNA barcoding in amphibians is certainly the species identification of larvae. Identification of tadpoles to species or even genus is an extremely time consuming task since it involves laborious rearing of eggs laid by well identified pair of adults or rearing of tadpoles collected from wild to juvenile stage and then to assign one of the species known to occur at the site (Vences et al., 2005). DNA barcoding clearly overcome these problems and to offer identification tools and has been applied in several studies to successfully identify tadpoles (Thomas et al., 2005). The present study is aimed to identify tadpoles collected from the wild by comparing COI gene sequences of both adult and tadpoles and to assess the usefulness of this method for rapid species identification at different life stages of an organism.

Item Type:	Article
Subjects:	Chemical Biology
Depositing User:	Central Library RGCB
Date Deposited:	30 Jul 2018 10:03
Last Modified:	30 Jul 2018 10:03
URI:	http://rgcb.sciencecentral.in/id/eprint/672

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