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Dhananjayan, Dhanasooraj and R , Ajay Kumar and Sathish, Mundayoor (2013) Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles. International journal of nanomedicine, 8. pp. 835-843. ISSN 1178-2013

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Abstract

Nano-sized hepatitis B virus core virus-like particles (HBc-VLP) are suitable for uptake by antigen-presenting cells. Mycobacterium tuberculosis antigen culture filtrate protein 10 (CFP-10) is an important vaccine candidate against tuberculosis. The purified antigen shows low immune response without adjuvant and tends to have low protective efficacy. The present study is based on the assumption that expression of these proteins on HBc nanoparticles would provide higher protection when compared to the native antigen alone. The cfp-10 gene was expressed as a fusion on the major immunodominant region of HBc-VLP, and the immune response in Balb/c mice was studied and compared to pure proteins, a mixture of antigens, and fusion protein-VLP, all without using any adjuvant. The humoral, cytokine, and splenocyte cell proliferation responses suggested that the HBc-VLP bearing CFP-10 generated an antigen-specific immune response in a Th1-dependent manner. By virtue of its self-adjuvant nature and ability to form nano-sized particles, HBc-VLPs are an excellent vaccine delivery system for use with subunit protein antigens identified in the course of recent vaccine research.

Item Type: Article
Uncontrolled Keywords: Mycobacterium tuberculosis, VLP, hepatitis B virus core particle, CFP-10, self-adjuvant, vaccine delivery
Subjects: Mycobacterium Research
Depositing User: Rgcb Library
Date Deposited: 14 Sep 2018 05:10
Last Modified: 14 Sep 2018 05:10
URI: http://rgcb.sciencecentral.in/id/eprint/685

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