Sajith , Raghunandanan and Ranjit, Ramachandran and Roshna , Lawrence Gomez and R , Ajay Kumar (2018) Rv0474 is a copper-responsive transcriptional regulator that negatively regulates expression of RNA polymerase β subunit in Mycobacterium tuberculosis. The FEBS journal. ISSN 1742-4658

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Abstract

We characterize Rv0474, a putative transcriptional regulatory protein of Mycobacterium tuberculosis, which is found to function as a copper-responsive transcriptional regulator at toxic levels of copper. It is an autorepressor, but at elevated levels (10-250 μm) of copper ions the repression is relieved resulting in an increase in Rv0474 expression. Copper-bound Rv0474 is recruited to the rpoB promoter leading to its repression resulting in the growth arrest of the bacterium. Mutational analysis showed that the helix-turn-helix and leucine zipper domains of Rv0474 are essential for its binding to Rv0474 and rpoB promoters, respectively. The mechanism of Rv0474-mediated rpoB regulation seems to be operational only in pathogenic mycobacteria that can persist inside the host.

Item Type:	Article
Uncontrolled Keywords:	Mycobacterium tuberculosis ; autoregulation; copper response; transcriptional regulator
Subjects:	Mycobacterium Research
Depositing User:	Central Library RGCB
Date Deposited:	28 Sep 2018 09:17
Last Modified:	28 Sep 2018 09:17
URI:	http://rgcb.sciencecentral.in/id/eprint/690

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