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Deepa, Indira and Shankara , Narayanan Varadarajan and Santhik , Subhasingh Lupitha and Asha , Lekshmi and Krupa , Ann Mathew and Aneesh, Chandrasekharan and Prakash , Rajappan Pillai and Ishaque Pulikkal , Kadamberi and Indu, Ramachandran and Hari , Sekar and Anurup, Kochucherukkan Gopalakrishnan and Santhoshkumar , TR (2018) Strategies for imaging mitophagy in high-resolution and high-throughput. European journal of cell biology, 97 (1). pp. 1-14. ISSN 1618-1298

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Abstract

The selective autophagic removal of mitochondria called mitophagy is an essential physiological signaling for clearing damaged mitochondria and thus maintains the functional integrity of mitochondria and cells. Defective mitophagy is implicated in several diseases, placing mitophagy as a target for drug development. The identification of key regulators of mitophagy as well as chemical modulators of mitophagy requires sensitive and reliable quantitative approaches. Since mitophagy is a rapidly progressing event and sub-microscopic in nature, live cell image-based detection tools with high spatial and temporal resolution is preferred over end-stage assays. We describe two approaches for measuring mitophagy in mammalian cells using stable cells expressing EGFP-LC3 - Mito-DsRed to mark early phase of mitophagy and Mitochondria-EGFP - LAMP1-RFP stable cells for late events of mitophagy. Both the assays showed good spatial and temporal resolution in wide-field, confocal and super-resolution microscopy with high-throughput adaptable capability. A limited compound screening allowed us to identify a few new mitophagy inducers. Compared to the current mitophagy tools, mito-Keima or mito-QC, the assay described here determines the direct delivery of mitochondrial components to the lysosome in real time mode with accurate quantification if monoclonal cells expressing a homogenous level of both probes are established. Since the assay described here employs real-time imaging approach in a high-throughput mode, the platform can be used both for siRNA screening or compound screening to identify key regulators of mitophagy at decisive stages.

Item Type: Article
Uncontrolled Keywords: Mitophagy Autophagy Super-resolution imaging High-throughput imaging Apoptosis
Subjects: Cancer Research
Depositing User: Rgcb Library
Date Deposited: 01 Oct 2018 07:26
Last Modified: 01 Oct 2018 07:26
URI: http://rgcb.sciencecentral.in/id/eprint/691

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