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Asha, Poorna C. and Resmi, M.S. and Soniya, E.V. (2013) In vitro Antioxidant Analysis and the DNA Damage Protective Activity of Leaf Extract of the Excoecaria agallocha Linn Mangrove Plant. Agricultural Chemistry, 1 (7,4:1-). ISSN ISBN: 978-953-51-1026-2

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Reactive oxygen species (ROS) are various forms of activated oxygen, which include free- radicals, e.g., superoxide anions (O2-), hydroxyl radicals (.OH), non-free-radical compounds (H2O2) and singlet oxygen (1O2), which can be formed by different mechanisms in living organisms. Oxidative damage of DNA molecules associated with electron-transfer reactions is an important phenomenon in living cells, which can lead to mutations and contribute to carcinogenesis and the aging processes. ROS species are considered as important causative factors in the development of certain diseases such as diabetes, stroke, arteriosclerosis, cancer and cardiovascular diseases, in addition to the aging process. Prior administration of antioxidant provides a close relationship between FRSA and the involvement of endocrinological responses, which help to reverse the effect [1, 2]. Plants are rich sources of phytochemicals such as saponin, tannin, flavanoids, phenolic and alkaloids, which possess a variety of biological activities including antioxidant potential. Antioxidants provide protection to living organisms from damage caused by uncontrolled production of ROS and concomitant lipid peroxidation, protein damage and DNA stand breaking. Natural antioxidants are in high demand for application as bio-pharmaceuticals, nutraceuticals and food additives. Terrestrial plants are considered potent sources of bioactive compounds and pharmacologically active compounds, however, little is known about the therapeutical potential of mangrove plants. Exploration of the chemical constituents of mangrove plants is necessary to find new therapeutic agents and this information is very important to the local community. Important reasons for studying the chemical constituents of mangrove plants are first, mangroves are a type of tropical forest that grows easily and has not as yet been widely utilized. Secondly, the chemical aspects of mangrove plants are very important because of the potential to develop compounds of agrochemical and medical value. The plants of the genus Excoecaria (family: Euphorbiaceae) comprise nearly 40 species which are distributed throughout the mangrove regions of tropical Africa, Asia and northwest Australia. The most widely reported mangrove species is Excoecaria agallocha Linn. The latex of this plant has been used as a purgative and abortifacient, as well as in the treatment of ulcers, rheumatism, leprosy and paralysis. The leaves and latex of this tree have been used as fish poison in India, New Caledonia and Malaysia. The bark and wood is used in Thailand as a remedy for flatulence. Recently, much attention has been paid to Excoecaria species due to their cytotoxic and anti-HIV activities [3]. In this study we investigated the antimicrobial and antioxidant potential of methanol extract of Excoecaria agallocha Linn leaf. The DPPH and the oxidative DNA damage preventive activity and antioxidant potential of the crude methanol extract and sequential hexane, water and methanol extract of E. agallocha Linn leaf were also investigated. We found that water extract of E. agallocha Linn was more effective and could scavenge reactive oxygen species (ROS) thus preventing DNA strand scission by •OH generated in the Fenton reaction on pCAMBIA1301 DNA.

Item Type: Article
Subjects: Plant Disease Biology
Depositing User: Central Library RGCB
Date Deposited: 08 Apr 2019 11:05
Last Modified: 12 Apr 2019 07:25
URI: http://rgcb.sciencecentral.in/id/eprint/721

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