Nisha, E.Thomas and Sasidharan , Shashikala and Suparna , Sengupta (2010) Cytoplasmic Gamma-Tubulin Complex From Brain Contains Nonerythroid Spectrin. Journal of cellular biochemistry, 110 (6). pp. 1334-41. ISSN 1097-4644

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Abstract

The newer member of the tubulin superfamily, g-tubulin, is known to mediate microtubule nucleation from the centrosome of eukaryotic cells with the aid of some other proteins. The major amount of g-tubulin is believed to be located in the centrosome before the onset of mitotic division. However, a considerable amount has been found in the cytoplasm in the form of a complex whose function is not well known. Microtubules are most abundant in brain tissues and brain microtubules have been extensively used in many in vitro studies. Thus, it is relevant to use brain tissue to characterize cytoplasmic g-tubulin complex. Here we show that cytoplasmic g-tubulin in brain tissues exists as a ring complex as in other tissues. Interestingly, along with the common members of the g-TuRC reported from several tissues and species, the purified brain cytoplasmic complex contains some high molecular weight proteins including a and b nonerythroid spectrin which are not found in other tissues. Immunohistochemical studies of brain tissue sections also show the co-localization of g-tubulin and spectrin. The possible implications have been discussed

Item Type:	Article
Uncontrolled Keywords:	g-TuRC; BRAIN; SPECTRIN; MICROTUBULES
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	22 May 2019 07:56
Last Modified:	22 May 2019 07:56
URI:	http://rgcb.sciencecentral.in/id/eprint/759

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