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Praveen , Kumar and Wilson A., Peter and Sabu , Thomas (2010) Rapid Detection of Virulence-Associated Genes in Environmental Strains of Vibrio cholerae by Multiplex PCR. Current Microbiology, 60 (3). pp. 199-202. ISSN 0343-8651

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Vibrio cholerae, the causative agent of cholera is ubiquitously distributed in aquatic environment particularly in coastal waters, estuaries, and rivers. In the present investigation, a multiplex PCR assay was developed for the detection of virulence-associated genes (rtxA, tcpA, ctxA, hlyA, and sto) in environmental isolates of V. cholerae. A total of 90 strains isolated from different environmental sources were screened for the presence of virulence-associated genes. Our results showed that this method represents a simple, cost effective, and robust tool for rapid detection of virulence-associated genes. This multiplex PCR can be used for examining prevalence of virulence-associated genes and hence will be useful for better understanding of epidemiology of environmental V. cholerae.

Item Type: Article
Uncontrolled Keywords: sabu@rgcb.res.in
Subjects: Environmental Biology
Depositing User: Rgcb Library
Date Deposited: 19 Jun 2019 08:55
Last Modified: 19 Jun 2019 08:55
URI: http://rgcb.sciencecentral.in/id/eprint/788

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