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Jamuna S. , Sreeja and Rince , John and K C., Sivakumar and E , Sreekumar and Suparna , Sengupta (2019) Binding of alpha-fodrin to gamma-tubulin accounts for its role in the inhibition of microtubule nucleation. ssengupta@rgcb.res.in, 593 (11). pp. 1154-1165. ISSN 1873-3468

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Non-erythroid spectrin or fodrin is present as part of the γ-tubulin ring complex (γ-TuRC) in brain tissue and brain derived cells. Here, we show that fodrin, which is otherwise known for providing structural support to the cell membrane, interacts directly with γ-tubulin within the γ-TuRC through a GRIP2-like motif. Turbidometric analysis of microtubule polymerization with nucleation-potent γ-TuRC isolated from HEK-293 cells that lack fodrin and the γ-TuRC from goat brain that contains fodrin shows inefficiency of the latter to promote nucleation. The involvement of fodrin was confirmed by the reduction in the microtubule polymerization efficiency of HEK-293 derived γ-TuRCs upon addition of purified brain fodrin. Thus, the interaction of fodrin with gamma-tubulin is responsible for its inhibitory effect on γ-tubulin mediated microtubule nucleation.

Item Type: Article
Uncontrolled Keywords: GRIP2 motif; fodrin; gamma-tubulin; microtubule; nucleation
Subjects: Cancer Research
Depositing User: Central Library RGCB
Date Deposited: 02 Aug 2019 09:57
Last Modified: 02 Aug 2019 09:57
URI: http://rgcb.sciencecentral.in/id/eprint/840

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