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Lekshmi , Narendrakumar and Mary , Theresa and Sivakumar, K C and Sabu, Thomas (2019) Tryptanthrin, a potential biofilm inhibitor against toxigenic Vibrio cholerae, modulating the global quorum sensing regulator, LuxO. Biofouling, 35 (10). pp. 1093-1103. ISSN 1029-2454

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Abstract

Cholera caused by the Gram-negative bacterium Vibrio cholerae still remains a major health burden in developing countries due to its high transmissibility and multidrug resistance. Alternative strategies are in quest to curtail the disease focusing on antivirulent approaches, such as biofilm inhibition, which make bacteria more susceptible to antibiotic therapies. The biofilm state is important for V. cholerae pathogenesis and its persistence in the environment. In the present study, tryptanthrin, a phytochemical, has been identified as possessing strong anti-biofilm activity at sub MIC (2 µg ml-1) against V. cholerae. LuxO was identified as the putative target of tryptanthrin by molecular docking and real time analysis. The phytochemical was identified as safe and possessed synergistic action with ciprofloxacin, a commonly used quinolone antibiotic to treat cholera. Collectively, the study establishes the first report on the anti-biofilm property of tryptanthrin by targeting LuxO, which could serve as a potential antivirulent therapy to combat V. cholerae infections.

Item Type: Article
Uncontrolled Keywords: Anti-biofilm; LuxO; MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); V. cholerae; quorum sensing;
Subjects: Cholera And Biofilm
Depositing User: Rgcb Library
Date Deposited: 31 Jan 2020 07:14
Last Modified: 31 Jan 2020 07:14
URI: http://rgcb.sciencecentral.in/id/eprint/944

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