Santhik , Subhasingh Lupitha and Leena , Chandrasekhar and Aparna Geetha , Jayaprasad and Aneesh , Chandrasekharan and Santhoshkumar, T.R. (2020) A reporter cell line for real-time imaging of autophagy and apoptosis. Toxicology Letters, 326. pp. 23-30. ISSN 0378-4274

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Abstract

Simultaneous detection of autophagy and apoptosis is important in drug discovery and signaling studies. Here we report, a real-time reporter cell line for the simultaneous detection of apoptosis and autophagy at single-cell level employing stable integration of two fluorescent protein reporters of apoptosis and autophagy. Cells stably expressing EGFP-LC3 fusion was developed initially as a marker for autophagy and subsequently stably expressed with inter-mitochondrial membrane protein SMAC with RFP fusion to detect mitochondrial permeabilization event of apoptosis. The cell lines faithfully reported the LC3 punctae formation and release of intermembrane proteins in response to diverse apoptotic and autophagic stimuli.

Item Type:	Article
Uncontrolled Keywords:	Apoptosis Autophagy Real-time imaging Drug screening
Subjects:	Cancer Research
Depositing User:	Central Library RGCB
Date Deposited:	17 Jun 2020 05:32
Last Modified:	17 Jun 2020 05:32
URI:	http://rgcb.sciencecentral.in/id/eprint/961

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