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Tara G. , Menon and E . V. , Soniya (2014) Isolation and characterization of salt-induced genes from Rhizophora apiculata Blume, a true mangrove by suppression subtractive hybridization. Current Science, 107 (4). pp. 650-655. ISSN 0011-3891

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Abstract

Mangroves are pla nts that can tolerate salinity equiv a- lent to that present in seawater . In the pr e sent study , we have attempted to isolate the genes upregulated at the early stage of salt stress response in Rhizophora apiculata Blume, a true mangrove, through suppression s ubtractive h ybridization technique. Several genes were isolated, which were then classified to nine fun c- tional categories , viz . m etabolism, p rotein degr a dation and folding, s econdary metabolism, c ell rescue and defen c e, t ransport facilitation, s ignal tran sduction, t ranscription and translation, p hotosynthesis and u n- classified genes of unknown function. The expression patterns of 12 of the genes thus obtained were studied at 6, 12 and 24 h time points of salinity stress using r eal - time PCR. Most genes were found to be upreg u - lated under salt stress and showed maximum upreg u - lation at the 6 h time point. Two of the genes studied were downregulated after 6 h, implying that the pa t- tern of gene expression varies with time of a p plication of stress. The genes thus i solated may be used to co n- fer the trait of salt tolerance to non - tolerant gen o- types, which can ultimately prove benef i cial for c rop improvement programme s

Item Type: Article
Uncontrolled Keywords: Gene Expression, Rhizophora Apiculata, Salt Stress, Suppression Subtractive Hybridization.
Subjects: Plant Disease Biology & Biotechnology
Depositing User: Rgcb Library
Date Deposited: 09 Jan 2017 04:23
Last Modified: 09 Jan 2017 04:23
URI: http://rgcb.sciencecentral.in/id/eprint/122

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