V. , Mallika and K.C., Sivakumar and E.V., Soniya (2011) evolutionary Implications and physicochemical Analyses of selected proteins of Type III polyketide synthase Family. Evolutionary bioinformatics online, 7. pp. 41-53. ISSN 1176-9343

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Abstract

Type III polyketide synthases have a substantial role in the biosynthesis of various polyketides in plants and microorganisms. Comparative proteomic analysis of type III polyketide synthases showed evolutionarily and structurally related positions in a compilation of amino acid sequences from different families. Bacterial and fungal type III polyketide synthase proteins showed <50% similarity but in higher plants, it exhibited >80% among chalcone synthases and >70% in the case of non-chalcone synthases. In a consensus phylogenetic tree based on 1000 replicates; bacterial, fungal and plant proteins were clustered in separate groups. Proteins from bryophytes and pteridophytes grouped immediately near to the fungal cluster, demonstrated how evolutionary lineage has occurred among type III polyketide synthase proteins. Upon physicochemical analysis, it was observed that the proteins localized in the cytoplasm and were hydrophobic in nature. Molecular structural analysis revealed comparatively stable structure comprising of alpha helices and random coils as major structural components. It was found that there was a decline in the structural stability with active site mutation as prophesied by the in silico mutation studies.

Item Type:	Article
Uncontrolled Keywords:	type III polyketide synthase, CHS, PKS, non-CHS, molecular phylogeny, chalcone synthase
Subjects:	Plant Disease Biology & Biotechnology
Depositing User:	Central Library RGCB
Date Deposited:	20 Apr 2017 08:50
Last Modified:	20 Apr 2017 08:50
URI:	http://rgcb.sciencecentral.in/id/eprint/346

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