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Sheeb, M.S. and Muneeb Hamz, K.H. and Asha, V.V (2014) Molecular diversities among Cardiospermum halicacabum Linn. populations in Kerala assessed using RAPD markers. Annals of Phytomedicine , 3 (2). pp. 87-92. ISSN 2 39 3 -9 88 5

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Abstract

A collection of Cardiospermum halicacabum Linn. plants was made from five different regions of Kerala to determine the extent of genetic variability using analysis at DNA level. As germplasm div ersiti es are not influ enced by the envir onmen t, RAP D (Ra ndom Amplifi ed Po lymorp hic DNA) provides different approach t owards the genotype ba sed classification of plants. T he present study was aimed to reveal the genetic polymorphism among five different populations of C. halicacabum . For this, the plants were collected from five different locations in Kerala. DNA was isolated from the leaves by CTAB (Cetyltrimethyl Ammonium Bromide) method and quantified spectrophotometrically. The isolated DNA was subjected to RAPD analysis by Polymerase chain reaction (PCR) using 120 different primers. Among the 120 primers, 25 primers yielded the best product for RAPD analysis. Unweighted Pair Grou p Method and Arithmetic Mean (UPGMA) dendrogram analysis resu lted in two clu sters su ch as the plants collected from T rivandru m, Kanyak u mari and Kollam were grou ped in one clu ster an d the remaining popu l ations from Alappuzha and Palakkad grouped in the other cluster. From the results, it has been observed that th e p lan ts col lected fro m five di fferen t l oca tio ns sho wed a low pol ymo rph ism. T he tot al polymorphism among the

Item Type: Article
Uncontrolled Keywords: Genetic diversity, polymerase chain reaction, germplasm, polymorphism, arbitrary primers, UPGMA
Subjects: Plant Disease Biology & Biotechnology
Depositing User: Rgcb Library
Date Deposited: 17 Jul 2017 10:22
Last Modified: 03 Jul 2019 09:11
URI: http://rgcb.sciencecentral.in/id/eprint/407

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