Ramesh J., Praseeja and Sreejith, P S. and V V. , Asha (2015) Studies on the apoptosis inducing and cell cycle regulatory effect of Cuscuta reflexa Roxb chloroform extract on human hepatocellular carcinoma cell line, Hep 3B. IJARNP, 8 (2). pp. 37-47. ISSN 1940-6223

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Abstract

Preliminary studies in our lab showed the anti-hepatocellular carcinoma (HCC) activity of Cuscuta reflexa in Hep 3B cell line. This study aims to detail the mechanism behind the anti-HCC effect of C. reflexa on HCC cell line. The chloroform extract of C. reflexa (CRCE) reduced the proliferation of Hep 3B cells in a dose and time dependent manner without showing much cytotoxic effect on non-hepatocyte cell lines, HEK 293 and RAW 264.7. C. reflexa induced apoptosis in Hep 3B cells as evidenced by DNA fragmentation, Annexin V-FITC apoptotic assay, PARP cleavage, Caspase activation etc. Treatment with this extract significantly increased the percentage of apoptotic cells. It also caused G1 arrest, associated with apoptotic induction. The active fractions present in CRCE were also isolated by TLC. Industrial relevance. Hepatocellular carcinoma (HCC) has gained major clinical interest because of its worldwide increasing incidence and carries a poor survival rate. A complete cure for this disease is not available today. C. reflexa is ethnomedically used for the treatment of various diseases especially jaundice. In this study we have evaluated the apoptosis inducing and associated cell cycle regulatory effect of CRCE in HCC cell line (Hep 3B). Results showed that CRCE is able to induce apoptosis in Hep 3B cells in a dose and time dependent manner through the intrinsic mitochondrial apoptotic pathway. CRCE is a potential candidate for further development as an anti-HCC drug. The HPTLC fingerprint profile of CRCE was analyzed and the active fraction was isolated. Further studies are needed to identify and characterize the isolated active fraction for the development of active anti-HCC drug.

Item Type:	Article
Uncontrolled Keywords:	Hepatocellular carcinoma; Hep 3B; Cuscuta reflexa; Apoptosis; Cell cycle arrest
Subjects:	Plant Disease Biology & Biotechnology
Depositing User:	Central Library RGCB
Date Deposited:	19 Feb 2018 07:15
Last Modified:	19 Feb 2018 07:15
URI:	http://rgcb.sciencecentral.in/id/eprint/513

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