V, Suresh and V, Sruthi and VV, Asha (2011) In vitro anti-inflammatory and anti-cancer activities of Cuscuta reflexa Roxb. Journal of ethnopharmacology, 134 (3). pp. 872-877. ISSN 1872-7573

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Abstract

To determine anti-inflammatory and anti-cancer activities of Cuscuta reflexa in cell lines (in vitro). MATERIALS AND METHODS: Anti-inflammatory activity of the water extract was analysed in vitro using lipopolysaccharide (LPS) induced inflammatory reactions in murine macrophage cell line RAW264.7. The expression of COX-2 and TNF-α genes involved in inflammation was analysed by SQ RT-PCR. EMSA was conducted to analyse the influence of the extract on NF-κB signalling. Anti-cancer activity was analysed on Hep3B cells by MTT assay, DAPI staining, annexin V staining and SQ-RT PCR analysis of BAX, Bcl-2, p53 and survivin. RESULTS: The extract down regulated LPS induced over expression of TNF-α and COX-2 in RAW264.7 cells; blocked NF-κB binding to its motifs and induced apoptosis in Hep3B cells as evidenced from MTT, DAPI staining and annexin V staining assays. The extract up regulated pro-apoptotic factors BAX and p53, and down regulated anti-apoptotic factors Bcl-2 and survivin. CONCLUSIONS: The study showed that Cuscuta reflexa inhibits LPS induced inflammatory responses in RAW264.7 cells through interplay of TNF-α, COX-2 and NF-κB signalling. It induced apoptosis in Hep3B cells through the up regulation of p53, BAX and down regulation of Bcl-2 and survivin.

Item Type:	Article
Subjects:	Plant Disease Biology & Biotechnology
Depositing User:	Central Library RGCB
Date Deposited:	02 May 2018 04:35
Last Modified:	02 May 2018 04:35
URI:	http://rgcb.sciencecentral.in/id/eprint/567

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