Jaya Kumar, Arjun and Balakrishna Pillai , Aneesh and Kumarapillai, Harikrishnan (2015) Sequencing and characterization of L-asparaginase (ansB) gene of Bacillus megaterium isolated from Western Ghats, Kerala, India. International Journal of Current Microbiology and Applied Sciences, 4 (6). 753- 760. ISSN 319-7706

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Abstract

L-asparaginase is a clinically approved bacterial therapeutic enzyme widely used in the treatment of Acute Lymphoblastic Leukaemia and Lymphosarcoma. In this study, periplasmic L-asparaginase producing bacteria were isolated from forest soil. The enzyme coding region (ansB gene) was amplified from the most productive isolate Bacillus megaterium PHB29, using group specific primers. The gene was cloned in pGEMT Easy vector and sequenced using automated DNA sequencer. The phylogeny of the gene was studied and compared with related sequences from NCBI public database. The secondary structure of the gene was analysed with SOPMA software. The antigenicity of the protein was predicted and compared with other commercially available enzymes using semi-empirical method. The present study indicates that soil bacteria are a promising source of commercially and therapeutically important drugs which are still unexplored.

Item Type:	Article
Uncontrolled Keywords:	L-asparaginase, Bacillus megaterium, 16S rRNA, pGEMT Easy vector
Subjects:	Environmental Biology
Depositing User:	Central Library RGCB
Date Deposited:	02 Jan 2017 06:22
Last Modified:	02 Jan 2017 06:22
URI:	http://rgcb.sciencecentral.in/id/eprint/101

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