Aneesh Kumar, A. and Ajith Kumar, G. S. and Gopika , Satheesh and Arun , Surendran and Mahesh , Chandran and Chandrasekharan, C. Kartha and Abdul, Jaleel (2021) Proteomics Analysis Reveals Diverse Molecular Characteristics between Endocardial and Aortic-Valvular Endothelium. Genes, 12 (7). ISSN 2073-4425

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Abstract

The variations in the protein profile of aortic-valvular (AVE) and endocardial endothelial (EE) cells are currently unknown. The current study's objective is to identify differentially expressed proteins and associated pathways in both the endothelial cells. We used endothelial cells isolated from the porcine (Sus scrofa) aortic valve and endocardium for the profiling of proteins. Label-free proteomics was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our proteomics analysis revealed that 29 proteins were highly expressed, and 25 proteins were less expressed in the valve than the endocardial endothelium. The cell surface markers, such as CD63, ICAM1, PECAM1, PROCR, and TFRC, were highly expressed in EE. In contrast, CD44 was highly expressed in AVE. The pathway analysis showed that metabolic process-related proteins and extracellular matrix-related proteins were enriched in valves. Differential enrichment of signaling pathways was observed in the endocardium. The hemostasis function-related proteins were increased in both endothelial cells. The proteins and pathways enriched in aortic-valvular and endocardial endothelial cells revealed the distinct phenotype of these two closely related cells.

Item Type:	Article
Uncontrolled Keywords:	aortic valvular endothelium, endocardial endothelium, label-free proteomics, pathway analysis
Subjects:	Cardiovascular Diseases And Diabetes Biology
Depositing User:	Central Library RGCB
Date Deposited:	05 Jan 2022 06:43
Last Modified:	05 Jan 2022 06:43
URI:	http://rgcb.sciencecentral.in/id/eprint/1064

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