Krishnakumar, K. A. and Muneeb Hamza, K.H and Asha, V.V. (2014) Antifibrotic activity of Phyllanthus maderaspatensis L. in Wistar rats. ANNALS OF PHYTOMEDICINE, 3(1). pp. 68-79. ISSN 2 27 8 -9 83 9

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Abstract

This study evaluates the effectiveness of Phyllanthus maderaspatensis L. against liver fibrosis in W istar rats. Hepatic fibrosis was experimentally indu ced in male rats with CCl 4 administration. Animals were then post treated with 200 mg/kg dose of P. maderaspatensis hexane extract (PmHE). Fibrosis was stu died u sing biochemical assays and histopathological techniques. The molecular mechanism of reversion of hepatic fibrosis by PmHE was studied , using semiquantitative RT-PCR, western blotting and gelatine zymography. PmHE was analyzed using high performance liqu id chromatography (HPLC) and high performance thin layer chromatography (HPTLC). PmHE was subjected to toxicity evalu ation in rats. Post treatment with PmHE almost normalized CCl 4 - induced fibrotic changes. PmHE treatment normalized serum parameters, reduced lipid peroxidation and maintained the normal level of reduced glutathione pool. The increased levels of alpha -smooth muscle actin (  SMA), collagen I and collagen III and mRNA transcripts were effectively reversed with PmHE post treatment. Histopathology of the liver confirmed its recovery from hepatic fibrosis. Moreover, the post treatment with PmHE , showed a significant redu ction in the hydroxyproline level in the liver. Fu rther, matrix metalloproteinase (MMP) activity of MMP-9 and MMP-2 was also reduced. HPTLC and HPLC analysis showed the presence of phyllanthin and quercetin in the extract. The mechanism of action of PmHE against hepatic fibrosis could be at least partly, due to the inhibition of oxidative stress. PmHE was found to be safe , based on the toxicity evaluation. Therefore, PmHE is a very promising antifibrotic agent.

Item Type:	Article
Uncontrolled Keywords:	Hepatic fibrosis, lipid peroxidation, hydroxyl proline, alpha-smooth muscle actin, oxidative stress
Subjects:	Plant Disease Biology
Depositing User:	Central Library RGCB
Date Deposited:	06 Jan 2017 03:45
Last Modified:	22 Apr 2019 09:09
URI:	http://rgcb.sciencecentral.in/id/eprint/117

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