Suresh, V. and N., Krishna Radhika and Asha , V.V. (2014) Anti-HBV activity of the different extracts from Phyllanthus rheedei Wight in cell culture based assay systems. Journal of ethnopharmacology, 156. 309- 315. ISSN 1872-7573

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Abstract

Ethnopharmacological relevance Phyllanthusrheedei Wight is a plant used by Muthuvan tribes of Kerala for treating liver related diseases. Materials and methods The different extracts of Phyllanthus rheedei were analysed on cell lines were viz, PLC/PRF, Hep3B, FLCII10 and HepG2215 for its anti-HBV property. The analysis was done through ELISA, SQRT-PCR and immuno blotting. The most active extract was then divided in to fractions using HPTLC and the most active fraction was further identified. Results From the screening experiments it was shown that the ethanol extract of this plant has the maximum activity in lowering the viral markers like HBsAg, HBV Core and HBV X protein and whole virions with comparatively lesser cytotoxicity. The dose responses of this particular extract were further established. Conclusions This study concluded that the ethanol extract of Phyllanthusrheedei is very much effective in preventing the multiplication of HBV at the cellular level. This study scientifically validated the tribal claim of the use of this plant for severe liver disorders.

Item Type:	Article
Uncontrolled Keywords:	Anti-HBV; ELISA; Hepatitis; Immuno blotting; Phyllanthus rheedei Wight; SQRT-PCR
Subjects:	Disease Biology
Depositing User:	Central Library RGCB
Date Deposited:	11 Jan 2017 04:48
Last Modified:	22 Apr 2019 10:14
URI:	http://rgcb.sciencecentral.in/id/eprint/134

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