Ashwanikumar , N and Nisha , Asok Kumar and Saneesh Babu , PS and Sivakumar, KC and Mithun Varghese , Vadakkan and Parvathi, Nair and Hema Saranya, I and Asha Nair, S and Vinod Kumar , GS (2016) Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil. International journal of nanomedicine, 2016 (11). 5583-5594. ISSN 1178-2013

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Abstract

The study shows that RADA-F6 peptide with pH-responsive self-assembling nature can be effectively used as a drug delivery system for the sustained release of a potent anticancer drug 5-fluorouracil (5-FU) at basic pH. As 5-FU contains the aromatic pyrimidine ring, RADA-F6 system is suitable for entrapping an aromatic drug due to effective π-π stacking with phenylalanine and be able to show better controlled release behavior. The stability and controlled release nature of RADA-F6 in different conditions followed by 5-FU entrapment at in silico conditions was confirmed by molecular dynamics simulation taking RADA-16 as control. Cytotoxicity of the drug-loaded RADA-F6 was measured by MTT assay and cellular uptake by confocal microscopy. Physicochemical characterization and further Western blot analysis and flow cytometric studies confirm that RADA-F6 can be successfully used as an efficient vector for pH-sensitive, controlled 5-FU delivery system.

Item Type:	Article
Uncontrolled Keywords:	scaffold, drug delivery, nanofibrous, aromatic
Subjects:	Chemical Biology
Depositing User:	Central Library RGCB
Date Deposited:	13 Mar 2017 07:27
Last Modified:	13 Mar 2017 07:27
URI:	http://rgcb.sciencecentral.in/id/eprint/287

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