Kannan, M and Saravanakumar, M and Abdul, Jaleel (2016) Proteomic analysis of the silkworm midgut during larval-pupal transition. ISJ - Invertebrate Survival Journal, 13. pp. 191-204. ISSN 1824-307X

Text Proteomic (ISJ-INVERTEBRATE).pdf Restricted to Registered users only Download (2343Kb)

Abstract

Metamorphosis is a key process in holometabolous insects since specific larval tissues, such as the midgut, the silk gland and the fat body, are remodeled into adult organs, or even disintegrate. However, the role of proteins and factors involv ed in the remodeling of these organs is still unclear. For this reason we undertook a proteomic study on the larval midgut of Bombyx mori to identify proteins whose expression significantly changes during larval-pupal metamorphosis and thus can have a role in this process. 2D-PAGE analysis showed upregulation or new expression of midgut proteins at 45 (pI 5.2), 29.8 (pI 6.11) and 20-kDa (pI 6-7) during the pupal st age. In addition zymogram analysis demonstrated the occurrence of protease activity at 37-kDa at the same developmental stage. By using MALDI-TOF-MS analysis the protein spots from 2D gel were identified as Hemolin, low molecular weight 30-kDa lipoproteins, HSP 20.8 and HSP 20.4, while the proteolytic band was identified as 37-kDa serine protea se. The evidence herein presented suggests that the identified proteins could be involved in the immune protection during the remodeling of silkworm midgut that occurs at larval-pupal transition and represents a platform of knowledge necessary for future functional studies

Item Type:	Article
Subjects:	Cardiovascular Diseases And Diabetes Biology
Depositing User:	Central Library RGCB
Date Deposited:	29 Mar 2017 06:51
Last Modified:	12 Apr 2019 08:09
URI:	http://rgcb.sciencecentral.in/id/eprint/331

Actions (login required)

View Item