Aneesh, B and Jaya Kumar, Arjun and Kavitha, T and Harikrishnan, K (2016) Production of Short Chain Length Polyhydroxyalkanoates by Bacillus megaterium PHB29 from Starch Feed Stock. nternational Journal of Current Microbiology and Applied Sciences, 5 (7). 816 -823. ISSN 2319 - 7706

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Abstract

Polyhydroxyalkanoates (PHAs) are accumulated in bacteria as an energy reserve and have attracted great scientific interest as a biodegradable, biocompatible alternative to conventional plastics. The study is focus ed on polyhydroxybutyrate (PHB) accumulation by an environmental bacterial isolate Bacillus megaterium PHB29 using starch as carbon feedstock. PHB accumulation in the isolate was visualized by staining techniques using Sudan Black B and Nile Red dyes. The biomass production as well as PHA accumulation in PHB29 was tested with starch as carbon source at different temperatures and found yielding 3.07 g/L cell dry mass with 73.46% (w/w) PHA accumulation at an optimum temperature 34 o C. The extracted polymer was subjected to 1 H NMR analysis and the spectrum showed signals for a methine group ( - CH - ), a methylene group ( - CH 2 - ) and a methyl group ( - CH 3 ) proving the polymer as poly - 3 - hydroxybutyrate (PHB). The ratio of starch to PHB conversion of this isolate was fo und to be better than other reported strains of Bacillus megaterium as well as other species among the genus Bacillus .

Item Type:	Article
Uncontrolled Keywords:	Poly - 3 - hydroxybutyrate, Bacillus megaterium, Amylase , Starch, 1H NMR .
Subjects:	Environmental Biology
Depositing User:	Central Library RGCB
Date Deposited:	21 Sep 2017 05:51
Last Modified:	12 Apr 2019 08:03
URI:	http://rgcb.sciencecentral.in/id/eprint/461

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