SANIL , GEORGE and V., PREEJI and V., RESHMY (2011) DEVELOPMENT OF SPECIES SPECIFIC MICROSATELLITE PRIMERS FOR FIVE AMPHIBIAN SPECIES OF WESTERN GHATS. Zoological Survey of India. pp. 289-291.

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Abstract

Genetic analysis of natural populations has allowed biologists to ask a wide variety of questions which previously could only be answered by extensive observation of the group in question. A number of genetic markers have proven to be useful, including mitochondrial DNA, Major Histocompatibility Complex loci, allozyme loci, and Variable Number of Tandem Repeat (VNTR) markers. VNTR markers are characterized by a core sequence which consists of a number of identical repeated sequences. They can be divided into two categories based on the repeat length: a) minisatellites, 15 -70 base pair (bp) repeats, which can be quite large, and microsatellites, 2-6 bp repeats, which seldom exceed 200 bp in total length. These sequences are regularly distributed throughout the genome and are found, on average, about every 10-20 Kb in Eukaryotes. At a given locus, there may be an important length polymorphism defined by the variable number of repeated motifs. They are usually flanked by conserved sequences which allow specific amplification of each microsatellite locus within a species. This conservative DNA allows the development of specific primers for PCR reactions that allow one to specifically amplifythe VNTR sequence between the conserved area on either side of the repeat.

Item Type:	Article
Subjects:	Chemical Biology
Depositing User:	Central Library RGCB
Date Deposited:	01 Aug 2018 06:07
Last Modified:	01 Aug 2018 07:04
URI:	http://rgcb.sciencecentral.in/id/eprint/673

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