Sumith , Retnamma Panicker and Kartha, C.C. (2010) Curcumin Attenuates Glucose-Induced Monocyte Chemoattractant Protein-1 Synthesis in Aortic Endothelial Cells by Modulating the Nuclear Factor- � B Pathway. Pharmacology, 85 (1). pp. 18-26. ISSN 1423-0313

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Abstract

High glucose (HG) induces monocyte chemoattractant protein-1 (MCP-1) synthesis in endothelial cells through nuclear factor kappaB (NFkappaB). We investigated whether curcumin, losartan and sodium salicylate (NaSal) attenuate HG-induced MCP-1 synthesis in rat aortic endothelial cells (RAECs) and explored the mechanism of action. METHODS: RAECs were stimulated with HG (25 mmol/l) for 24 h in the presence or absence of curcumin, losartan, NaSal or NFkappaB inhibitor, Bay 11-0782. The MCP-1 protein and mRNA levels were determined by enzyme-linked immunosorbent assay and real-time reverse transcriptase-polymerase chain reaction, respectively. Nuclear translocation of NFkappaB subunit p65 and NFkappaB DNA-binding activity was studied using confocal microscopy and electrophoretic mobility shift assay, respectively. RESULTS: A significant increase in the synthesis of MCP-1 protein and mRNA (2-fold) was observed in HG-primed RAECs compared to control glucose (5.5 mmol/l). Curcumin (30 micromol/l) significantly decreased HG-induced MCP-1 protein (74%) and mRNA (53%) synthesis. There was no inhibition of HG-induced MCP-1 protein secretion by losartan and NaSal. In HG-stimulated RAECs, curcumin attenuated the nuclear translocation of p65 and decreased the NFkappaB DNA-binding activity.

Item Type:	Article
Uncontrolled Keywords:	Monocyte chemoattractant protein-1CurcuminEndothelial cellHigh glucoseLosartanSodium salicylateNuclear factor-ĸB
Subjects:	Cardiovascular Diseases And Diabetes Biology
Depositing User:	Central Library RGCB
Date Deposited:	02 Sep 2019 11:00
Last Modified:	02 Sep 2019 11:00
URI:	http://rgcb.sciencecentral.in/id/eprint/857

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