Shoeb, M. and Malini, Laloraya and Pradeep, G. Kumar (2010) Progesterone-induced reorganisation of NOX-2 components in membrane rafts is critical for sperm functioning in Capra hircus. Andrologia, 42 (6). pp. 356-65. ISSN 1439-0272

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Abstract

Spermatozoa produce increasing levels of reactive oxygen species (ROS) during development. We examined the functional organisation of NOX-2 subunits in developing spermatozoa and its involvement in ROS generation. We found NOX-2 components associated predominantly with caveolin-rich microdomains of sperm head and mid piece membranes. Capacitation in vitro enriched NOX-2 components within the caveolin-positive sperm membrane rafts. Progesterone, but not 17β-oestradiol, brought about loss of caveolin and extensive reorganisations in NOX-2 distribution on sperm head microdomains and accompanying activation in the levels of superoxide. The sensitivity of superoxide production by spermatozoa to methyl-β-cyclodextrin confirms that NOX-2 function in spermatozoa is tightly dependent on raft organisation. We conclude that progesterone-induced reorganisation of NOX-2 components in sperm membrane rafts is critical for sperm functioning.

Item Type:	Article
Uncontrolled Keywords:	Acrosome reaction—capacitation—NOX— raft—spermatozoa—superoxide
Subjects:	Molecular Reproduction
Depositing User:	Central Library RGCB
Date Deposited:	19 Sep 2019 09:25
Last Modified:	19 Sep 2019 09:25
URI:	http://rgcb.sciencecentral.in/id/eprint/872

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