Smrithi , Krishnan R and Neethu, Puthumadathil and Amina H., Shaji and Santhosh Kumar, K. and Gayathri , Mohan and Mahendran , K R. (2020) Designed alpha-helical barrels for charge-selectivepeptide translocation†. Chemical Science. ISSN 1473-7604

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Abstract

Synthetic alpha-helix based pores for selective sensing of peptides have not been characterized previously.Here, we report large transmembrane pores, pPorA formed from short synthetic alpha-helical peptides oftunable conductance and selectivity for single-molecule sensing of peptides. We quantified the selectivetranslocation kinetics of differently charged cationic and anionic peptides through these synthetic poresat single-molecule resolution. The charged peptides are electrophoretically pulled into the poresresulting in an increase in the dissociation rate with the voltage indicating successful translocation ofpeptides. More specifically, we elucidated the charge pattern lining the pore lumen and the orientationof the pores in the membrane based on the asymmetry in the peptide-binding kinetics. The salt and pH-dependent measurements confirm the electrostatic dominance and charge selectivity in controllingtarget peptide interaction with the pores. Remarkably, we tuned the selectivity of the pores to chargedpeptides by modifying the charge composition of the pores, thus establishing the molecular andelectrostatic basis of peptide translocation. We suggest that these synthetic pores that selectivelyconduct specific ions and biomolecules are advantageous for nanopore proteomics analysis andsynthetic nanobiotechnology applications

Item Type:	Article
Subjects:	Chemical Biology
Depositing User:	Central Library RGCB
Date Deposited:	24 Nov 2020 07:21
Last Modified:	24 Nov 2020 07:21
URI:	http://rgcb.sciencecentral.in/id/eprint/988

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