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Aswati , R. Nair and George, Thomas (2012) Functional genetic diversity at nucleotide binding site (NBS) loci: Comparisons among soft rot resistant and susceptible Zingiber taxa. Biochemical Systematics and Ecology, 44. pp. 196-201. ISSN 0305-1978

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Abstract

Fundamental problem confronting ginger production is uniform susceptibility of all cultivars to soft rot disease caused by Pythium spp. Resistance gene candidate sequences (RGCs) belonging to non-TIR (Toll-Interleukin receptor) NBS-LRR (Nucleotide Binding Site-Leucine Rich Repeat) class of resistance (R) genes was previously identified from cultivated and wild Zingiber taxa including Zingiber zerumbet, a putative resistance donor. Efficiency and sensitivity of SSCP (Single-strand conformation polymorphism) analysis over RFLP (Restriction fragment length polymorphism) analysis in discriminating Pythium susceptible and resistant Zingiber accessions is being described here. Zingiber RGCs detected 29.5% polymorphism in Z. zerumbet, 12.34% in Zingiber cernuum and no polymorphism in the analyzed ginger cultivars. Results are discussed in terms of their mode of propagation and R gene evolution in the wild and cultivated accessions. Four RGCs designated ZzP226, ZcP28, ZoP26 and ZcP623 that yielded Z. zerumbet specific bands constitute important genomic tools for isolation of full-length R-genes from resistant germplasm.

Item Type: Article
Uncontrolled Keywords: Ginger; Zingiber zerumbet; Resistance gene analogs; SSCP; Genetic diversity
Subjects: Plant Disease Biology & Biotechnology
Depositing User: Rgcb Library
Date Deposited: 13 Feb 2017 07:09
Last Modified: 04 Jul 2019 08:02
URI: http://rgcb.sciencecentral.in/id/eprint/228

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