Aswati , R. Nair and George, Thomas (2012) Functional genetic diversity at nucleotide binding site (NBS) loci: Comparisons among soft rot resistant and susceptible Zingiber taxa. Biochemical Systematics and Ecology, 44. pp. 196-201. ISSN 0305-1978

Text functional genetic diversity(biochem systematics and ecology).pdf Restricted to Registered users only Download (182Kb)

Abstract

Fundamental problem confronting ginger production is uniform susceptibility of all cultivars to soft rot disease caused by Pythium spp. Resistance gene candidate sequences (RGCs) belonging to non-TIR (Toll-Interleukin receptor) NBS-LRR (Nucleotide Binding Site-Leucine Rich Repeat) class of resistance (R) genes was previously identified from cultivated and wild Zingiber taxa including Zingiber zerumbet, a putative resistance donor. Efficiency and sensitivity of SSCP (Single-strand conformation polymorphism) analysis over RFLP (Restriction fragment length polymorphism) analysis in discriminating Pythium susceptible and resistant Zingiber accessions is being described here. Zingiber RGCs detected 29.5% polymorphism in Z. zerumbet, 12.34% in Zingiber cernuum and no polymorphism in the analyzed ginger cultivars. Results are discussed in terms of their mode of propagation and R gene evolution in the wild and cultivated accessions. Four RGCs designated ZzP226, ZcP28, ZoP26 and ZcP623 that yielded Z. zerumbet specific bands constitute important genomic tools for isolation of full-length R-genes from resistant germplasm.

Item Type:	Article
Uncontrolled Keywords:	Ginger; Zingiber zerumbet; Resistance gene analogs; SSCP; Genetic diversity
Subjects:	Plant Disease Biology & Biotechnology
Depositing User:	Central Library RGCB
Date Deposited:	13 Feb 2017 07:09
Last Modified:	04 Jul 2019 08:02
URI:	http://rgcb.sciencecentral.in/id/eprint/228

Actions (login required)

View Item