Sindhu Chandrika, Unni and Padmanabhan Jayanthikumari , Vivek and Thakidiyil Thankappan, Maju and Rintu Thundiyil , Varghese and E V, Soniya (2012) Molecular cloning and characterization of fruit specific promoter from Cucumis sativus L. American Journal of Molecular Biology, 2012 (2). 132-139 . ISSN 2161-6663

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Abstract

The isolation and characterization of fruit specific promoters are critical for the manipulation of nutri- tional value and agronomic quality of fruits by ge- netic engineering and also opened a new era in edible vaccine technology. Expansins are proteins that in- duce loosening of individual plant cells by disrupting the non-covalent interactio ns between cellulose and hemicellulose microfibrils and hence have role in growth programs including fruit ripening. We report the identification of an expansin gene ( CsExp ) from Cucumis sativus that exhibits high levels of mRNA abundance and is specifically expressed in ripened fruit. The promoter region of CsExp also contains elements responsible for its fruit specific expression. Transient expression studies of the CsExp promoter were conducted with partic le bombardment, followed by GUS histochemical assay and real time PCR. CaMV35S promoter was used as the positive control in all these experiments. Clear fruit specificity was observed for CsExp promoter in all the experiments. Thus CsExp promoter from Cucumber is a good can- didate to target expression of the foreign genes to en- gineer fruit specific traits.

Item Type:	Article
Uncontrolled Keywords:	Cucumber; Expansin; Fruit Specific Promoter; Transient Expression; Glucuronidase
Subjects:	Plant Disease Biology & Biotechnology
Depositing User:	Central Library RGCB
Date Deposited:	23 Feb 2017 04:42
Last Modified:	23 Feb 2017 04:42
URI:	http://rgcb.sciencecentral.in/id/eprint/259

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